The Basque Resource for Electron Microscopy (BREM) provides access to high-end instrumentation and expertise in high-resolution cryo-electron microscopy (Cryo-EM) to national and international researches, from academia and industry. Located at Instituto Biofisika (UPV/EHU, CSIC) in the scientific park of the UPV/EHU in Leioa, and supported primarily by the Department of Education and the Innovation Fund of the Basque Government, with additional support from Fundación Biofísica Bizkaia through the Basque Excellence Research Centres program, BREM focuses on structure determination of vitrified biological specimens – macromolecules and their complexes, organelles, cells and tissue – with the goal of understanding the structural basis of biological processes and the pathogenesis of human diseases. BREM also supports structure-based drug discovery efforts and the development of advanced disease therapies. Through the Recovery, Transformation and Resilience Plan of the Spanish Government, BREM has been selected to participate in a multi-disciplinary project on personalized medicine alongside prominent research institutions from several autonomous regions in Spain.

Thanks to an ambitious IKUR 2030 strategy by the Basque Government to foster research excellence in key scientific and technological niches, BREM has installed the first ThermoFisher Titan Krios G4 transmission electron microscope for structural biology in Spain. The Krios G4 is paired with Gatan´s BioContinuum Imaging Filter and a K3 direct electron detector device, as well as a phase plate, a Falcon III direct electron detector device and Ceta-D CMOS camera also from ThermoFisher.

BREM has highly-qualified staff to operate the facility and assist users with three-dimensional structure determination using all major cryo-EM techniques available for structural biology.

: Krios G4 operational