Instrumentation
Krios G4
ThermoFisher Titan Krios G4 transmission electron microscope, paired with Gatan’s BioContinuum Imaging Filter and a K3 direct electron detector device. The system also includes a phase plate, a second Falcon III direct electron detector device and Ceta-D CMOS camera.
| Magnification | K3 Nominal pixel size | K3 Calibrated pixel size |
|---|---|---|
| 81 000 X | 1.1 Å | 1.0552 Å |
| 105 000 X | 0.85 Å | 0.8238 Å |
| 130 000 X | 0.67 Å | 0.6462 Å |
| 165 000 X | 0.50 Å | 0.5054 Å |
| 215 000 X | 0.385 Å | 0.3979 Å |
Cryo-FIB/SEM
Thermo Fisher’s Aquilos 2 Plus: A Cutting-Edge Cryo-FIB/SEM System with Integrated iFLM for Ultra-Precise Cryo-Lamella Sample Preparation for In-Situ Cryo-Electron Tomography.
Glow discharger
ELMO glow discharger for TEM grids.
EM GP2
Leica EM GP2 single-side blotting automated plunge freezer.
Vitrobot Mark IV
ThermoFisher Vitrobot Mark IV double-side blotting automated plunge freezer.
EM ICE
Leica EM ICE automated high pressure freezer.
Computational Resources
Processing server with 8 GPUs
Hot storage server with 130 TB SSD
Warm storage server with 500 TB HDD