Instrumentation

Krios_G4

Krios G4

ThermoFisher Titan Krios G4 transmission electron microscope, paired with Gatan’s BioContinuum Imaging Filter and a K3 direct electron detector device. The system also includes a phase plate, a second Falcon III direct electron detector device and Ceta-D CMOS camera.

MagnificationK3 Nominal pixel sizeK3 Calibrated pixel size
81 000 X1.1 Å1.0552 Å
105 000 X0.85 Å0.8238 Å
130 000 X0.67 Å0.6462 Å
165 000 X0.50 Å0.5054 Å
215 000 X0.385 Å0.3979 Å

Cryo-FIB/SEM

Thermo Fisher’s Aquilos 2 Plus: A Cutting-Edge Cryo-FIB/SEM System with Integrated iFLM for Ultra-Precise Cryo-Lamella Sample Preparation for In-Situ Cryo-Electron Tomography.

This Cryo-DualBeam Microscope merges the high-resolution capabilities of a Scanning Electron Microscope (SEM) with the rapid and accurate specimen milling provided by a Focused Ion Beam (FIB) system. Additionally, the Aquilos 2 features an Integrated Fluorescent Light Microscope (iFLM) Correlative System, enhancing its functionality by enabling the capture of fluorescent images. This allows for precise identification and detailed examination of specific areas within biological samples.

Glow discharger

ELMO glow discharger for TEM grids.

EM GP2

Leica EM GP2 single-side blotting automated plunge freezer.

Vitrobot Mark IV

ThermoFisher Vitrobot Mark IV double-side blotting automated plunge freezer.

EM ICE

Leica EM ICE automated high pressure freezer.

Computational Resources

Processing server with 8 GPUs

Hot storage server with 130 TB SSD

Warm storage server with 500 TB HDD